diff --git a/awscli/customizations/s3/subcommands.py b/awscli/customizations/s3/subcommands.py
index bd3f60f16479..aed4a8a4f414 100644
--- a/awscli/customizations/s3/subcommands.py
+++ b/awscli/customizations/s3/subcommands.py
@@ -459,7 +459,7 @@
 }
 
 CHECKSUM_ALGORITHM = {
-        'name': 'checksum-algorithm', 'choices': ['CRC64NVME', 'CRC32', 'SHA256', 'SHA1', 'CRC32C', 'SHA512', 'XXHASH64', 'XXHASH3', 'XXHASH128'],
+        'name': 'checksum-algorithm', 'choices': ['CRC64NVME', 'CRC32', 'SHA256', 'SHA1', 'CRC32C', 'SHA512', 'XXHASH64', 'XXHASH3', 'XXHASH128', 'MD5'],
         'help_text': 'Indicates the algorithm used to create the checksum for the object.'
 }
 
diff --git a/tests/unit/customizations/s3/test_subcommands.py b/tests/unit/customizations/s3/test_subcommands.py
index 82d85bf6a25f..2389f8f7d25e 100644
--- a/tests/unit/customizations/s3/test_subcommands.py
+++ b/tests/unit/customizations/s3/test_subcommands.py
@@ -753,6 +753,14 @@ def test_validate_checksum_algorithm_download_error(self):
             cmd_params.add_paths(paths)
             self.assertIn('Expected checksum-algorithm parameter to be used with one of following path formats', cm.msg)
 
+    def test_validate_checksum_algorithm_md5_upload(self):
+        paths = [self.file_creator.rootdir, 's3://bucket/key']
+        parameters = {'checksum_algorithm': 'MD5'}
+        cmd_params = CommandParameters('cp', parameters, '')
+        cmd_params.add_paths(paths)
+        self.assertEqual(cmd_params.parameters['checksum_algorithm'], 'MD5')
+
+
     def test_validate_checksum_algorithm_sync_download_error(self):
         paths = ['s3://bucket/key', self.file_creator.rootdir]
         parameters = {'checksum_algorithm': 'CRC32C'}